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News: Waters Atlantis HPLC Columns - UK Authorised Distributor

Article Date(s):07/25/2011 - 07/25/2011

Atlantis Columns

Atlantis T3 Columns are ideal for diverse analyte mixtures

Providing balanced retention of polar and non-polar molecules, Atlantis® T3 columns are the culmination of more than three decades of experience and understanding in stationary-phase design. With selective optimization of pore diameter, C18-ligand density and endcapping, Atlantis T3 columns provide paramount retention for polar compounds, while exhibiting industry leading peak shape and efficiency for all analytes as well as exceptional chemical stability across a wide pH range.

The brilliant performance provided by T3 bonding technology can be achieved across HPLC and UPLC® Technology platforms. ACQUITY UPLC® HSS T3 columns incorporate the same T3 bonding and endcapping technology as Atlantis T3 columns, thus providing the same versatility and performance independent of your separation platform.


Click Here To Order Your Waters Atlantis HPLC Columns

Atlantis HILIC Silica Columns for enhanced retention of polar compounds

Hydrophilic Interaction Chromatography (HILIC) is a chromatographic technique that can be used to improve retention of very polar species that are poorly retained by reversed-phase chromatography.

Atlantis HILIC Silica columns employ an organic-rich mobile phase to induce retention, therefore providing increased mass spectrometry response, making HILIC an attractive technique for bionalytical scientists.


Guide to Retaining Polar Compounds


Atlantis® HILIC Silica columns improve retention of very polar analytes and metabolites that are poorly retained by reversed-phase chromatography by Hydrophilic Interaction.

Benefits:

  • Improved mass spectrometry response
  • Direct compatibility with solid-phase extraction (SPE) eluates
  • Complementary selectivity to reversed-phase HPLC

Retention Mechanisms in Hydrophilic Interaction Chromatography

HILIC retention mechanisms are a complex combination of partitioning, ion-exchange and hydrogen bonding, resulting in enhanced retention for polar analytes.

The Standard for HILIC Column Retention

For the best combination of peak shape and retention, Atlantis HILIC Silica columns set the industry standard for HILIC separations.

Atlantis® T3 columns are universal, silica-based, reversed-phase C18 columns that provide balanced retention of polar and hydrophobic molecules.

T3

Selective optimization of pore diameter, C18-ligand density and endcapping, enable Atlantis T3 columns to provide paramount retention for polar compounds, while exhibiting industry leading peak shape and efficiency for all anayltes as well as exceptional chemical stability across a wide pH range.  The advantages of T3 bonding technology are extended to UPLC® technology in ACQITY UPLC® HSS T3 columns. 

For reversed-phase applications, Atlantis T3 columns should be your first choice when developing separations of polar (and non-polar) compounds.

Superior Retention and Peak Shape Under 100% Aqueous Conditions

10 mM Ammonium Formate, pH 3.0, 30 °C

Atlantis T3 columns provide 100% aqueous compatibility, superior polar compound retention and excellent peak shape for acids, neutrals and bases.

Superior Low pH Stability

T3 bonding allows Atlantis T3 columns to provide long column lifetimes under harsh, low pH conditions.

Improved Peak Shape for Bases at pH 7

When compared to other reversed-phase "polar compound retention columns", Atlantis T3 columns provide superior pH 7 peak shape.

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