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Inertsil HILIC HPLC Columns

Inertsil HILIC

  • Unique Selectivity and Elution Order for Polar Compounds
  • Polar Compounds show Increased Retention with Increased Organic Content
  • Greatly reduced ion suppression in LCMS
  • Vast database of chromatographic applications available to assist in method development
  • Very low operating back pressure

What is HILIC mode? Hydrophilic Interaction Chromatography

Analysis of highly polar and basic compounds has been problematic since the beginning of HPLC. Some polar compounds are so difficult to analyze that they require high aqueous buffer content, even on the most inert reverse phase HPLC columns, resulting in imperfect peak shape and impaired MS sensitivity.

HILIC was developed as an alternative to reverse phase chromatography for polar compounds, and has also shown great promise in SFC. The elution order in HILIC mode is typically the opposite of what it might be in reverse phase mode. In HILIC, the HIGHER the organic concentration, the GREATER the retention of more polar compounds.

Advantages of HILIC mode

High organic content mobile phases result in low operating back pressure and ability to run at high flow rates

A high organic solvent concentration of the mobile phase will lead to a high sensitivity LC/MS analysis.

Reduced Ion suppression effect in LC/MS analyses.

More precise quantitation of polar compounds owing to improved peak shape and the rapid elution of hydrophobic sample components.

    
   

Particle
Size(s)
Particle
Shape
Surface
Area
Pore
Size
Pore
Volume
Silica
Purity
Bonded
Phase
End-
Capping
Carbon
Load
pH
Range*
3, 5, 8 µmSpherical450 m2/g100Å1.05 mL/g99.999%alkyl diolYes22 - 7.5
* Inertsil phases are known to provide excellent results and long column life at pH levels of 9-10. However, optimum column may be achieved at a pH between 2 and 7.5.


Item Name Product Image Price

GL Sciences Inertsil Hilic 5µm, CapillaryEX-Nano Column 0.05 x 50mm - 5020-15024

GL Sciences Inertsil Hilic 5µm, CapillaryEX-Nano Column 0.05 x 50mm - 5020-15024
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GL Sciences Inertsil Hilic 3µm, CapillaryEX-Nano Column 0.05 x 50mm - 5020-15025

GL Sciences Inertsil Hilic 3µm, CapillaryEX-Nano Column 0.05 x 50mm - 5020-15025
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GL Sciences Inertsil Hilic 5µm, CapillaryEX-Nano Column 0.05 x 150mm - 5020-15074

GL Sciences Inertsil Hilic 5µm, CapillaryEX-Nano Column 0.05 x 150mm - 5020-15074
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GL Sciences Inertsil Hilic 3µm, CapillaryEX-Nano Column 0.05 x 150mm - 5020-15075

GL Sciences Inertsil Hilic 3µm, CapillaryEX-Nano Column 0.05 x 150mm - 5020-15075
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GL Sciences Inertsil Hilic 5µm, CapillaryEX-Nano Column 0.05 x 250mm - 5020-15124

GL Sciences Inertsil Hilic 5µm, CapillaryEX-Nano Column 0.05 x 250mm - 5020-15124
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GL Sciences Inertsil Hilic 3µm, CapillaryEX-Nano Column 0.05 x 250mm - 5020-15125

GL Sciences Inertsil Hilic 3µm, CapillaryEX-Nano Column 0.05 x 250mm - 5020-15125
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GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 50x4.6mm - 5020-03274

GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 50x4.6mm - 5020-03274
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GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 50x4.6mm - 5020-03275

GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 50x4.6mm - 5020-03275
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GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 50x4.0mm - 5020-03374

GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 50x4.0mm - 5020-03374
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GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 50x4.0mm - 5020-03375

GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 50x4.0mm - 5020-03375
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GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 50x3.0mm - 5020-03474

GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 50x3.0mm - 5020-03474
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GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 50x3.0mm - 5020-03475

GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 50x3.0mm - 5020-03475
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GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 50x2.1mm - 5020-03574

GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 50x2.1mm - 5020-03574
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GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 50x2.1mm - 5020-03575

GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 50x2.1mm - 5020-03575
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GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 10x4.0mm - 5020-03674

GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 10x4.0mm - 5020-03674
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GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 10x4.0mm - 5020-03675

GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 10x4.0mm - 5020-03675
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GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 33x4.6mm - 5020-04174

GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 33x4.6mm - 5020-04174
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GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 33x4.6mm - 5020-04175

GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 33x4.6mm - 5020-04175
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GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 33x4.0mm - 5020-04274

GL Sciences Inertsil Hilic 5µm, Conventional Guard Column 33x4.0mm - 5020-04274
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GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 33x4.0mm - 5020-04275

GL Sciences Inertsil Hilic 3µm, Conventional Guard Column 33x4.0mm - 5020-04275
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