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ChromaCon FPLC Systems

ChromaCon AG is a private Life Science tool company located in Zürich, Switzerland. ChromaCon implements novel cyclic and continuous chromatography process principles and corresponding operating software and control on chromatography hardware systems. The resulting systems outperform standard batch chromatography systems by providing significant (30-50%) CAPEX and OPEX cost savings and allow faster processing as well as enabling scalable chromatographic solutions for large scale purification applications. ChromaCon’s proprietary processes position it to be a leader in cyclic and continuous downstream manufacturing operations.

The Contichrom® equipment platform consists of two lab-scale/prep-scale equipment lines, as

  • The Medium-pressure Contichrom® CUBE and Contichrom® CUBE Combined for protein chromatography marketed by ChromaCon and its distributors
  • The High-pressure Contichrom® Lab-10 for small molecule purification marketed by Knauer

The Contichrom® CUBE line, consisting of two modules with complementary functions incorporates all process modes. Batch, sequential batch, CaptureSMB®, MCSGP and N-Rich® and also has the AutomAb® function.

The Contichrom® Lab-10 incorporates batch and MCSGP process capabilities.

Both equipment lines employ twin-columns with cyclic and continuous process capabilities.

For GMP and non-GMP scale-up, ChromaCon has partnered with LEWA Process technologies (LPT), offering customized pilot and process –scale equipment. ChromaCon has implemented its twin-column process technology into LPT’s hardware and operating software.

  • Contichrom® CUBE and Contichrom® CUBE Combined are modular twin–column lab-scale equipment for biopharmaceutical protein process development applications, operated at medium pressure

Click here to view the NEW Cube System brochure :

  • Contichrom® Lab-10 and Prep-100 systems are twin–column lab-prep scale equipment for small and large molecules, operated at high pressure. This equipment line is marketed through a partner company Knauer.

Click here to view the Lab-10 System Brochure :

  • Both equipment lines are operated by the ChromIQ® software, offering multiple process choices for single and twin-column operations allowing the use of all chromatographic process principles with a single piece of equipment.

Click here to view the NEW Twin Column Chromatography Process Portfolio :

For GMP scale-up equipment, ChromaCon has license agreements with LEWA and LEWA Process Technologies for implementation of ChromaCon’s proprietary process principles at GMP pilot and process scale.

Contichrom® CUBE

The Contichrom® CUBE is a dedicated powerful purification system for biopharmaceuticals such as monoclonal antibodies, designed for medium pressure preparative chromatography applications.

  • Run batch, cyclic or continuous processes with the user-friendly ChromIQ® automation software that will provide you automated process optimization and control.
  • Get 50% more yield in protein purification with the same target purity.
  • Develop economic monoclonal antibody capture processes saving up to 50% on Protein A resin costs.
  • Incremental addition of process capabilities.

The Contichrom® CUBE equipment line consists of two modules with complementary functions that incorporate all process modes. It supports batch, sequential batch, CaptureSMB®, MCSGP and N-Rich® processes and also has the AutomAb® function. The CUBE equipment line employs twin-columns with cyclic and continuous process capabilities.

The CUBE Combined, consists of the CUBE and the CUBE+ modules.

The first module, the Contichrom® CUBE, is a stand-alone module, having batch, integrated batch, CaptureSMB® and AutomAb® process and control capabilities and being ideally suited for monoclonal antibody purification using a standard platform purification process. CaptureSMB® is used to efficiently capture proteins on affinity resins, such as Protein A or affinity resins for tagged proteins. AutomAb® is a program helping to optimize the capture step automatically. The polishing step is done by normal batch chromatography employing either step or gradient elution. Two single batch polishing steps can be integrated in one run, employing the twin column setup.

Contichrom® CUBE module

The second module, the Contichrom® CUBE+ is an extension of the first CUBE module and cannot be operated stand-alone. It has additional process capabilities for high resolution polishing, employing MCSGP and N-Rich® processes. These processes operated as twin-column processes provide significantly higher yield without compromising target purity. If you have aggregates that you want to separate from your product you can use the MCSGP process as an SMB process to binary separate product from aggregates with high efficiency and yield. N-Rich® is a tool for isolating minor compounds, such as product-related impurities or biomarkers. These processes combined with small-particle resins allow to obtain almost analytical resolution with preparative chromatography.

Contichrom® CUBE+ module

For scaling up to GMP pilot/process scale, our licensing partner LEWA and LEWA Process Technologies will implement batch, CaptureSMB® and MCSGP processes into customized hardware skids with operating software, providing best-in-class GMP process solutions. Their skid production site in Devens, MA welcomes visitors. (www.lewapt.com)

The CUBE Combined, consists of the CUBE and the CUBE+ modules.

ChromIQ® Software

The ChromIQ® software runs the twin column Contichrom® CUBE preparative protein chromatography equipment. It contains all the familiar processes and tools for separation and purification with an intuitive, user-friendly interface.

It is full of useful tools to help you run more efficient batch processes, easy step-by-step wizards to help you convert to the more efficient Contichrom® Processes and tools like AutomAb® to run an optimized CaptureSMB® process and let you take advantage of the benefits of the Process Portfolio.

The following ChromIQ® software wizards, are delivered with the CUBE modules:

Batch wizard, CaptureSMB® wizard, AutomAb® wizard, MCSGP wizard, N-Rich wizard

ChromIQ® also has an expert operating mode for deeper control and a full range of analysis and reporting tools.

The software contains all essential elements for 21CFR part 11 compliance:

  • Pre-defined user groups, administrators, R&D and production users
  • Rights management for individual user groups
  • User accounts are password protected
  • Logging with time stamp and user name

Electronic signature with checksum of log and measurement files.

Here you can download several process animations which help to understand the working principle of the Contichrom® Platform

  • Contichrom®   SMB

CaptureSMB®

How does CaptureSMB work?

Monoclonal antibodies (mAbs) and Fc fusion proteins are purified using platform processes involving a capture step, and subsequent polish and filtration steps. A fast capture step is critical to retaining product integrity and processing of large feed volumes in reasonable times. Breakthrough curves of single-column batch processes show that only 50-60% of the resin material is used.

CaptureSMB is a two Column Periodic Counter Current Chromatography (2C PCC) process. CaptureSMB thus uses a twin column setup and alternates the feed input and elution steps between the columns (see powerpoint animation below). Thus, all possible product from a continuous feed stream is captured and all of the available affinity resin, for example Protein A, is efficiently used.

Click here to download the CaptureSMB process principle presentation :

The CaptureSMB technology is patented by ChromaCon and can be run on both the Contichrom CUBE and the Contichrom CUBE Combined. CaptureSMB processes can be created and optimized towards throughput or resin cost savings using the AutomAb software wizard.

 

Please choose and click on the links below for further ChromaCom product information :




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